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13. MRS medium

Peptone

10.0

g/L

Meat extract

5.0

g/L

Yeast extract

5.0

g/L

Glucose

20.0

g/L

Tween 80

1.0

g/L

CaCO3

5.0

g/L

Sodium acetate

5.0

g/L

Diammonium citrate

2.0

g/L

MgSO4.7H2O

0.58

g/L

MnSO4.4H2O

0.28

g/L

(Agar

16.0

g/L)

 

Distilled water is added to obtain the desired volume. pH is adjusted to 7.0

14. NA (Nutrient Agar) medium

Peptone

5.0

g/L

Meat extract

2.0

g/L

NaCl

1.0

g/L

Agar

20.0

g/L

 

Distilled water is added to obtain the desired volume. pH is adjusted to 7.0 - 7.5

2. Ashby medium

Glucose

20.0

g/L

K2HPO4

0.2

g/L

MgSO4.7H2O

0.2

g/L

NaCl

0.2

g/L

K2SO4

0.1

g/L

CaCO3

5.0

g/L


Distilled water is added to obtain the desired volume. pH is adjusted to 7.0 - 7.5

21. YM medium

Glucose

10.0

g/L

Peptone

5.0

g/L

Yeast extract

3.0

g/L

Malt extract

3.0

g/L

(Agar

20.0

g/L)


Distilled water is added to obtain the desired volume. pH is adjusted to 6.2

23. YS (Yeast extract-Soluble starch) medium

Yeast extract  

2.0

g/L

Soluble starch 

10.0

g/L

Malt extract

3.0

g/L

(Agar

18.0 g/L)

Distilled water is added to obtain the desired volume. pH is adjusted to 6.2

24. PDA (Potato-Dextrose Agar) Medium

Potato, peeled and cut

200.0

g/L

Glucose

10.0

g/L

Agar

15.0

g/L


Boil potatoes for 20 min. Strain off the pieces of potatoes through a fine sieve. Add glucose and agar, and boil until dissolved.
Distilled water is added to obtain the desired volume. pH should be adjusted to 5.4-5.6.

25. Malt Extract agar medium

Malt extract  

20.0

g/L

Glucose

20.0

g/L

Peptone

1.0

g/L

Agar

18.0

g/L


Distilled water is added to obtain the desired volume

28. Marine agar

Protease peptone 

2.0

g/L

Yeast extract

2.0

g/L

Phyto peptone

1.0

g/L

Sodium thiosulfate 0.4  g/L
Sodium thiosulfite 1 g/L

Ferric citrate

0.08

g/L

Distilled water 

250

mL

Sea water 750 mL

pH is adjusted to 7.4-7.8

33. Tryptic Soy Agar medium

Tryptone

17.0

g/L

Soytone

3.0

g/L

Dextrose

2.5

g/L

NaCl 

5.0 g/L

K2HPO4

2.5

g/L

Agar

15

g/L


Distilled water is added to obtain the desired volume. pH is adjusted to 7.1-7.5.

6. Fred and Waksman medium

Mannitol

10.0

g/L

K2HPO4

0.5

g/L

MgSO4

0.2

g/L

NaCl

0.2

g/L

CaCO3

1.0

g/L

Yeast Extract

5.0

g/L

Agar

20.0

g/L

Congo red solution

1

mL/L


Distilled water is added to obtain the desired volume.
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